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KMID : 0903619990400050595
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Journal of the Korean Society for Horticultural Science
1999 Volume.40 No. 5 p.595 ~ p.597
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Gamma-ray Sensitivity of Chrysanthemum Cultured In Vitro
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한봉희/Han, Bong Hee
신학기/예병우/구대회/고재영/이수영/송희섭/Shin, Hak Ki/Yae, Byeong Woo/Goo, Dae Hoe/Ko, Jae Young/Lee, Su Young/Song, Hi Sup
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Abstract
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Gamma-ray sensitivity of Chrysanthemum ´Bongwhang´ shoots grown in vitro was investigated. Lethality of the buds increased according to the increasing γ-ray quantity. LD_(50) of ´Bongwhang´ was 40 gray. As the result of 40 gray γ-ray irradiation to 6 cultivars, lethality of ´Bongwhang´ was 50%, ´Subangryuk´ 64.0%, ´Puma´ 62.5%, ´Hijung´ 50.5%, ´Herman de Boon´ 53.8%, and ´Seolpoong´ 7.5%. Days for bud sprouting of γ-ray irradiated ´Bongwhang´ were within 9 days in 10-25 gray irradiation with little differences among irradiated quantities, but rapidly increased to more than 18 days in 30-50 gray irradiation. In M₁V₃ generation, proliferation by 50 gray irradiation was 5.1 times while 10.6 times of control. The higher γ-ray quantity resulted in lower shoot proliferation ratio. Average proliferation ratio of γ-ray irradiated shoots was 2.7 times in M₁V₂ generation and 6.4 times in M₁V₃ generation. Mutants of nonbranching and flower shape were appeared by 17.5 gray, leaf and stem mutants by 15.0 gray, and early flowering by 25.0 gray.
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KEYWORD
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